Agricultural and Biological Research

Plant growth regulators' effects on various pomegranate cv. Sindhuri explants cultured in vitro

Ravi Kumar*

The nodal segment, shoot apex, and leaf explant of the Sindhuri cultivar of Punica granatum were planted on MS Medium supplemented with varying concentrations of auxins and cytokinins single or in combination in order to induce calluses and shoot buds. The best media to induce shoot buds in nodal segment explants was found to be MS media supplemented with 2.0 mg/l BAP. The highest degree of shoot bud induction in shoot apex explants was observed with MS media having 2.5 mg/l BAP. Rich callus induction was accomplished in shoot apex explants by utilizing MS medium supplemented with 2.0 mg/l BAP. The best media for inducing callus in leaf explants was found to be MS media supplemented with 1.0 mg/l BAP+1.0 mg/l NAA.