GET THE APP

Effect of culture media and photoperiod on in vitro culture | 105009
An International Journal

Agricultural and Biological Research

ISSN - 0970-1907
RNI # 24/103/2012-R1

Abstract

Effect of culture media and photoperiod on in vitro culture of pomegranate cv. Sindhuri

Ravi Kumar*

To observe how various photoperiod regimes and culture media affect in vitro cultures, particularly with regard to direct shoot growth, callus induction and organogenesis. A variety of six cultural media (MS medium, Nitsch and Nitsch, WPM, Schenk and Hildebrant medium, White's medium, and Khudson Solution C) and photoperiod regimes (16:8, 14:10, 12:12, and 8:16) were used to plant the nodal segment, shoot apex, and leaf explant of the Sindhuri cultivar of pomegranate. The leaf explant supplemented with the most responsive level (1.0 mg/l BAP+1.0 mg/l NAA) showed the largest induction of callus. In shoot apex explants treated with WPM at the most responsive dosage (2.5 mg/l BAP), maximum shoot bud induction was also noted. For de novo shoot regeneration from callus culture, WPM supplemented with 1.0 mg/l BAP+2.0 mg/l NAA was shown to be the most effective. WPM and MS media were determined to be the most effective among all the media for callus induction, shoot bud proliferation, and de novo shoot formation. All the photoperiod regimes investigated, the 14:10 hour regime was shown to be the most effective for shoot bud induction, callus differentiation, and de novo shoot development.

Journal Hilights
  • Abstracting and indexing in renowned databases
  • Expert editorial team
  • High quality articles
  • High visibility
  • International readership
  • Language editing
  • Membership
  • Online manuscript submission and tracking system
  • Rapid peer review process
  • Reprints of published articles
Journal is Indexed in:
  • BIOSIS Previews and Zoological Record which are part of the life sciences in Web of Science (WOS)
  • Euro Pub
  • Google Scholar
  • MIAR
  • Publons
Journal Flyer
Flyer
Google Scholar Citation Report
Citation image
Peer Review Process Check
Publon image
Top