Herbs are enriched in bioactive compounds and utilized to treat a variety of ailments in the indigenous medicinal system. This research looks at the water-cleaning potential of Moringa stenopetal seed extract. Water samples were mainly collected in the Wuseta and Teklehaimanot rivers of Debre Markos, Ethiopia. Both samples of water have been purified and then treated using dried seed extract. The treated samples collected were analyzed using bacterial study via utmost of the possible techniques. The introduction of aluminium sulfate as a flocculant reduced the pH of the water from 7.32 to 3.95, but the pH of the seed extract water remained constant. Usage of 0.036 g/L of M. stenopetal reduced water turbidity from 131.11 NTU to 16.82 NTU (87.30%) and even from 210 NTU to 33.68 NTU (84.25%), respectively, in the Weseta and Tekelehymanot samples from the river. The acetone extraction had the highest antibacterial activity against Salmonella enterica with 19.20 mm, whereas E. coli (clinical isolate) had the lowest value, at 8.75 mm on the methanol source. Acetone extract's most common MIC value was 7.50 mg/mL. At extremely low concentrations, the acetone extract is the most efficient in inhibiting and killing Salmonella typhi (clinical isolate).The seed acetone extracts showed significant antibacterial action. It determines that the seed extracts can manage and diminish waterborne bacterial infections. The research assists people in Ethiopia and throughout the world who can still not acquire or do not have clean drinking water available to them.