The present study was conducted to evaluate combine effect by using Rhizobium sp and Agrobacterium sp as a biofertilizer on nodule formation and growth of chick-pea plant and to study the efficiency of seed inoculation for nitrogen fixation. Rhizobium sp and Agrobacterium sp effect was studied using Cicer arietinum and controlled condition. Rhizobium sp and Agrobacterium sp were isolated from root nodules of Cicer arietinum, sterilized, prepared serial dilutions, incubated then applied on Cicer arietinum seeds and also on controlled seeds, pot experiments were conducted to evaluate the effect of bacterized seeds and controlled seeds. Therefore, it can be concluded that combined biofertilizer (Rhizophium sp and Agrobacterium sp) application was the most effective in terms of yield in Cicer arietinum cropping pattern and can be used to reduce the use of chemical fertilizers for sustainable crop production in terms of yield and soil fertility as well as environmental safety. However, more and intense systematic studies are required to provide better understanding of biofertilizer use in making crop production more profitable income generating.