Agricultural and Biological Research

Analyzing the impact of growth hormones on in vitro propagation of Anacyclus pyrethrum

Gajanand Modi* and Ravi Kishan Soni

Traditional medicine makes use of the wild species Anacyclus pyrethrum, also known as A. pyrethrum, which belongs to the Asteraceae family. In order to commence the cultivation of Anacyclus pyrethrum, the study employed 4 to 5 cm immature nodal shoot segments. Antioxidant therapy solved the early issues of leaching and browning when the axillary meristem was activated on MS media with 2.0 mg/L of 6-Benzylaminopurine (BAP), resulting in profuse shoot growth. 0.5 mg/L 6-benzylaminopurine and kinetin amplified cultures were subculturing on Murashige and Skoog (MS) medium. Root induction was achieved on half-strength MS medium with 2.0 mg/L Indole-3-Butyric Acid (IBA) and 0.2% activated charcoal and ex vitro rooting with pulse administration of 200.0 mg/L indole-3-butyric acid demonstrated efficacy. The tissue culture approach shown to be reliable and appropriate for large-scale greenhouse cultivation of akarkara plants.