Solanum virginianum L belonging to family Solanaceae selected for decolourisation of Reactive Turquoise Blue H5G dye. In vitro grown cultures of S.virginianum were able to remove more than 50% dye concentration up to 110 mg/l. comparative analysis of biochemical and antioxidant study showed more activity in treated plants as compared to untreated plants. The phytotoxicity study demonstrated the non-toxic nature of degraded metabolites. Use of such non-edible yet medicinal plant for phytoremediation is discussed.